Line Profile

Overview

The Line Profile module can compute intensity profile from an image along a line or curve.

Panels and their use

• Parameters:

  • Input volume: The intensity profile will be computed from voxel values of this image.

  • Input line: The image will be sampled along this line or curve. Click and select Create new line or Create new curve and the click in the views.

  • Line resolution: Specifies how many samples will be taken from the image along the input line.

• Advanced:

  • Output intensities table: Intensity values will be stored in this table.

  • Show plot: If this option is enabled then any updates will automatically display the intensity plot in the view layout.

  • Output plot series: This series will be set up to plot the intensity values.

  • Plot proportional distance (%): If this option is enabled then distance values are relative (in percentage) along the line.

• Peak detection:

  • Peaks output table: Peak detection results will be stored in this table.

  • Minimum peak width: Minimum width of the peak. Use higher values to reject small peaks detected due to image noise.

  • Height for width measurement: Height at which the peak width is measured. 50% computes full width at half maximum. Larger value means that the height is measured near the top of the peak.

  • Peak is maximum: If checked then peaks are local maximum values. If unchecked then peaks are local minimum values.

• Compute intensity profile: Click the button to compute the intensity profile. Check the checkbox in the button to enable auto-update, i.e., automatically update the intensity profile when the line or any parameter is changed.

Contributors

Andras Lasso (PerkLab), Natalie Johnston (Revvity), James Butler (Revvity), Mike Bindschadler, Adam Aji, chir.set, Csaba Pinter (Ebatinca)

Acknowledgements

This work was was partially funded by Revvity and Children’s Hospital of Philadelphia.